Knowledge of 184 physicians on the procedure of the biological crossmatch at the beginning of bloodtransfusions has been studied. Special formalized questionnaire was established for answers to the 4 questions aboutnumber of drops in 1 ml of blood; about actions during the biological crossmatch; about diagnostic monitoringmethods; about records. An ambiguous understanding of the regulatory requirements has been found. The maximumfrequency of the most popular answer to the questions was 49.1%, 44.2%, 74.5% and 47.9%, respectively. It isadvisable to change the text of the standards in order to harmonization of scientifically sound international practice.